Feb 25, 2022 · Intracellular fully reduced HMGB1 (fr-HMGB1), with three conserved cysteines containing thiol groups, can form a complex with CXCL12 and promote the migration of immune cells.

May 29, 2018 · Then, we analyzed the HMGB1 localization by immunofluorescence staining. The HMGB1 in non-irradiated and irradiated tumor cells was mainly localized in the nucleus, whereas in irradiated...

Sep 11, 2020 · To confirm the link between inflammasome activation and HMGB1 release, we performed immunofluorescence on BMDM treated with LPS alone or in combination with nigericin.

In this review, we summarize the novel mechanisms underlying the regulation of active HMGB1 secretion by innate immune cells, and highlight potential HMGB1-targeting therapeutic strategies for the treatment of infection- and injury-elicited inflammatory diseases. Figure 1.

High-mobility group box 1 (HMGB1) protein is a highly abundant protein that can promote the pathogenesis of inflammatory and autoimmune diseases once it is in an extracellular location. This translocation can occur with immune cell activation as ...

Nov 2, 2017 · Extracellular HMGB1 can interact with pattern recognition receptors to stimulate pro-inflammatory and immunostimulatory pathways. Here, we developed a screening assay to identify pharmacological agents endowed with HMGB1 releasing properties.

Jan 1, 2019 · Extracellular HMGB1 can interact with pattern recognition receptors to stimulate innate immune responses. Here, we describe a discovery pipeline for the identification of pharmacological agents endowed with HMGB1 releasing properties.

Jan 1, 2019 · Immunofluorescence. Immunofluorescence (IF) microscopy can also be utilized to analyze the spatial distribution and subcellular expression of HMGB1 with high specificity and sensitivity.

Sep 1, 2019 · In this study, we explored how the soluble abundant high-mobility group box 1 protein (HMGB1) affected DC activation and retention within lung cancers, and in which way the resultant interferon-γ (IFN-γ) further enhanced DC maturation and accumulation.

Jun 24, 2021 · Using simplified Cross‐Linking and Immuno‐Precipitation and functional tests, we show that HMGB1 is also a bona fide RNA‐binding protein (RBP) binding hundreds of mRNAs. It presents an interactome rich in RBPs implicated in senescence regulation.