The first step was to remove the genomic DNA (gDNA) using gDNA eraser ... and four technical replicates were set for each biological replicates, qRT-PCR was performed to detect interference efficiency ...

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Consequently, this approach provides a more faithful representation of gene expression ... For genotyping, genomic DNA was extracted from tail tips and assayed using polymerase chain reaction (PCR) ...

Detection of the bacterial 16SrRNA genes in clinical specimens by PCR and microarray analysis can be used to accurately diagnose neonatal sepsis. This method has a higher sensitivity and ...

Even dead bacteria, it seemed, could share their genes. Almost all bacterial genomes show evidence of past LGT events, and the phenomenon is known to have profound effects on microbial biology. This ...

To investigate how Cas9 gene editing affected T cells, the researchers targeted the first exon of the TRAC locus, which encodes the T cell receptor on chromosome 14. As expected, DNA sequencing ...

It is a very valuable tool for assessing the quality of your DNA and RNA samples before proceeding with expensive Next Generation Sequencing (NGS), Microarray and Gene Expression experiments ... the ...

Genomic DNA was extracted using the phenol-chloroform method ... In order to develop a system for detecting low-presence allele variants of the CCR5 gene using digital droplet PCR, we set up a series ...

Evidently the most decisive definition of molecular response and molecular recurrence is critical to plan safe discontinuation of TKI; but as shown, a definition based only on qRT-PCR can result in ...

Knowledge of the exact distribution of meiotic crossovers (COs) and gene conversions (GCs ... validated by Sanger sequencing of PCR products of all four tetrad offspring. All PCRs were done on ...