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Antibodies are one of the most specific tools for the detection and capture of molecular targets­­. The right antibody can play a key role in revealing the presence, quantity, dynamics, and even ...
2012). Chromosomal aberrations studies were conducted using the immunofluorescence protocol and a primary antibody anti-αtubulin (Sigma Aldrich, DM1A) and anti-CREST at a 1:200 dilution. The secondary ...
The current standard for this is using fluorescently labeled secondary antibodies targeted to primary antibodies of interest to be visualized with fluorescent or confocal microscopes. One of the most ...
For immunofluorescence staining, we incubated secondary antibodies for 1 h while avoiding light at room temperature. For histomorphometric measurement, the tibial subchondral bone area was determined ...