C&EN17d
Streamlined High-Throughput Data Analysis Workflow for Antibody-Drug Conjugate Biotransformation Characterization
Common fixed modifications included C-terminal lysine loss and N-terminal glutamine to pyroglutamate conversion. Common IgG glycans were selected as possible glycans. For disulfide connections, any ...
Frontiers1y
The impact of immunoglobulin G N-glycosylation level on COVID-19 outcome: evidence from a Mendelian randomization study
Although observational studies demonstrate that changes in IgG glycome composition are closely related ... These structures may exhibit features such as core-fucose, bisecting N-acetylglucosamine ...
GitHub2y
jesseduffield/lazygit: simple terminal UI for git commands
Rant time: You've heard it before, git is powerful, but what good is that power when everything is so damn hard to do? Interactive rebasing requires you to edit a goddamn TODO file in your editor? Are ...
eLife2y
Double NPY motifs at the N-terminus of the yeast t-SNARE Sso2 synergistically bind Sec3 to promote membrane fusion
The structure shows a previously uncharacterized binding site for Sec3 at the N-terminus of Sso2, consisting of two highly conserved triple residue motifs (NPY: Asn-Pro-Tyr). We further reveal that ...
eLife6y
A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
We cloned immunoglobulin G (IgG) variable domains from cryopreserved hybridoma cells and input them into an integrated pipeline for expression and validation of functional R-mAbs. To improve ...
Frontiers6y
Sialylated Autoantigen-Reactive IgG Antibodies Attenuate Disease Development in Autoimmune Mouse Models of Lupus Nephritis and Rheumatoid Arthritis
Pro- and anti-inflammatory effector functions of IgG antibodies ... Fc glycan consists of four N-acetylglucosamines (GlcNAcs) and three mannoses, which can be further modified with fucose, bisecting ...