Next-generation DNA sequencing has started a revolution ... Once the fragments are attached, a single labeled nucleotide and polymerase mix are added across the surface. The polymerase ...

A single DNA nucleotide, the base unit of the human genome, is a tiny, tiny thing. Each one is made up of only 30 atoms (plus or minus a few, depending on the base), making it much too small to be ...

As shown in Figure 2, the 5'-phosphate group of the new nucleotide binds to ... which involved exposing replicating DNA to a short "pulse" of isotope-labeled nucleotides and then varying the ...