The CRISPR-Cas9 system offers groundbreaking genome editing capabilities, yet off-target cleavage raises concerns about ...

We propose a general quantitative framework of polymer bending that includes both the weak and strong bending regimes on the same footing, based on a single general physical ... For double-stranded ...

M13mp18 single-stranded DNA (7249) was purchased from New England Biolabs ... stacking and cross-stacking forces, hydrogen bonding, backbone connectivity, and electrostatic repulsion. Here, we ...

The sgRNA expression vector backbone was obtained ... sgRNA-fusion protein association and DNA binding in cells. In practice, signal detection with this probe could potentially be used as a precursor ...

Biolayer interferometry binding assay for determination of kinetics and affinity of UNG protein to various single- and double-stranded DNA templates was performed on an Octet Red384 instrument ...

Amount of RNA was quantified with Nanodrop (Thermo Fisher; Cat #ND2000CLAPTOP), and 2 μg of total RNA was retro-transcribed using Transcriptor First Strand ... at single-nucleotide resolution. The ...

AP lyase activity was optimal when using single-stranded DNA substrates containing adenine under reaction conditions of 0.5 mM AC buffer at pH 4 and supplemented with 20 μg/mL BSA. Leveraging the ...

Researchers have revealed new details about the CRISPR-Cas5-HNH/Cascade complex, a variant of the type I-E CRISPR-Cas system, ...

Relying on a single strand of hair, LinusBio is launching its diagnostic aid for autism spectrum disorder—with a test designed to chart a child’s environmental exposures over time. The company ...

"Country music has always been and is a backbone of the American culture and the music scene," Kathy Fielder, CEO of Thrive by Kathy Fielder, told Fox News Digital. Dr. Lyric Mandell, co-founder ...