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PCR was carried out using Taq DNA Polymerase (Sigma Chemical ... PCR products were separated by electrophoresis on an ethidium bromide-containing agarose gel-and photographed under UV light.
Afterward, the amount of microbial DNA was measured with a Qubit device, and its quality was evaluated through 1% agarose gel electrophoresis. DNA concentration ≥ 20 ng/μL and volume ≥ 20 μL (total ...
1.5 kb and 500 bp DNA fragments were placed in two independent lines along with another line for 1 kb Ladder (NEB, N232 ... linear DNA was quantified over a 2-h time course using agarose gel ...
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The 10–kb, PCR–amplified fragment was detected by gel electrophoresis as a chief band (Fig. 2). The correctly assembled DNA was a chief species and could be easily separated by electrophoresis ...
Department of Clinical Laboratory of Sir Run Run Shaw Hospital, College of Biosystems Engineering and Food Science, Zhejiang University School of Medicine, Hangzhou 310058, People’s Republic of China ...
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23 The quantification of extracted DNA was estimated by electrophoresis on 1% (m/v) (Ludwig Biotec, Alvorada, Brazil). All the identified strains were evaluated for their ability to produce fructose ...
Department of Chemistry, York University, 4700 Keele St, Toronto, ON M3J 1P3, Canada Centre for Research on Biomolecular Interactions, York University, 4700 Keele St, Toronto, ON M3J 1P3, Canada ...