Antibodies are one of the most specific tools for the detection and capture of molecular targets­­. The right antibody can play a key role in revealing the presence, quantity, dynamics, and even ...

The current standard for this is using fluorescently labeled secondary antibodies targeted to primary antibodies of interest to be visualized with fluorescent or confocal microscopes. One of the most ...

A “no primary antibody” control was performed for every experiment and sample. Immunofluorescence staining panels were performed with appropriate primary and secondary antibody overnight at 4C or for ...

Their respective horseradish peroxidase-coupled secondary antibodies were obtained from KPL (Gaithersburg ... The changes in mucus barrier integrity were assessed by immunofluorescence analysis.