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Here we use cryogenic electron microscopy to determine the structure of TbAQP2 from Trypanosoma brucei, bound to either the substrate glycerol or to the sleeping sickness drugs, pentamidine or ...

The assay was run for 1 h in an incubator at 25°C (PHCbi, MIR-154-PE) with minimal light from outside. The assay was conducted using Bonsai software real-time analysis. Red illumination of 35 and 140 ...