Prior to the development of PCR in the 1980s, the primary method for producing many copies of a gene was a relatively time-consuming process known as DNA cloning. This technique involved insertion ...

Long-range PCR 6 Complex genomic DNA (up to 20kb) and protein-coding DNA templates (30kb and more) can be amplified using long-range PCR. 6 A combination of highly processive thermostable DNA ...

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Methylation of cytosines in CpG dinucleotides is an important regulator of gene expression in the ... for bisulfite treatment of human genomic DNA. The PCR is performed with one of the PCR primers ...