Advanced light microscopy techniques are giving scientists a new understanding of human biology and what goes wrong in ...

Figure 1. A number of fluorescent probes can be excited with a single laser (blue line). In confocal microscopy, laser excitation light is raster scanned across the sample to induce the fluorescence ...

Looking at the dynamics of living cells is very challenging compared to looking at cells that have been fixed in stasis, even though live cells are far more relevant to scientific study. As such, ...

Fluorescence lifetime imaging microscopy (FLIM) offers optically encoded data about live cells processes. In contrast, atomic force microscopy (AFM) provides mechanical and nanometer-resolved surface ...

Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking. PLoS ONE, 2011; 6 (7): e22678 DOI: 10.1371/journal.pone.0022678 ...

Introduction The development of super-resolution fluorescence microscopy techniques that can achieve resolutions in the nanometer range, and thus enable the investigation of sub-cellular structures in ...

Figure 1. (A) Real 2D image of a C2C12 cancerous cell immunostained using Alexa Fluor 488 antibodies and acquired with a N-SIM super-resolution microscope system (Nikon Instruments) equipped with CFI ...

Now Alexa Mattheyses and colleagues at the University of Alabama at Birmingham and Emory University are filling that knowledge gap, using a sophisticated fluorescence microscopy imaging called STAR ...

Endocytosis-derived extracellular vesicles (EVs), which can be as small as 100 nm, are useful for disease prediction. However, very small EVs are below the optical diffraction limit and are difficult ...