Annealing: Short DNA fragments bind complementary regions of the template to begin new strand synthesis. Extension: Nucleotides are added to the primers, which serve as extension points to continue ...
universal primer can take over when the forward primer is used up; (3) since the annealing temperature of the fluorescent dye-labeled M13(-21) primer is only 53°C, the last eight PCR cycles ...
PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...
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