Maximum Intensity Projection is a common tool to represent 3D biological imaging data in a 2D space, but it creates artefacts. Here the authors develop Smooth Manifold Extraction, an ImageJ/Fiji ...

“That’s the name we gave it because it’s more precise than 2D imaging, but it doesn’t create any 3D images. That’s why we call it 2.5D,” she said. Plotting Pick-Heights and Sending ...