The technology involves three steps. First, reverse transcriptase makes DNA copies of the mRNA, using a chimeric DNA-RNA oligo-dT primer. Next, DNA polymerase copies the DNA, producing a ...

1| Add 0.001–100 ng of poly(A) + mRNA or 0.01–1 μg of total RNA into each of three microcentrifuge tubes. Adjust the volumes to 9 μl with water. Denature the RNA by heating at 75 °C for 5 ...

Gene expression studies by quantitative reverse-transcription PCR (qRT-PCR) can be challenging ... First, the kit enables the user to amplify the mRNA and convert it to cDNA directly from a ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...