The recommended final concentration is 1-10 percent. Formamide weakens base pairing and increases primer annealing specificity, thus enhancing PCR amplification for templates with high GC content.

since the annealing temperature of the fluorescent dye-labeled M13(-21) primer is only 53°C, the last eight PCR cycles should be run with an annealing temperature of 53°C (the same result can be ...

PCR is very precise and can be used to amplify ... The mixture is then cooled so that the primers anneal, or bind, to the DNA template. At this point, the DNA polymerase begins to synthesize ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

You could just put everything in the tube, close it, and cycle the temperature, and PCR would occur.” Additionally, raising the annealing and elongation temperatures increased primer binding ...

Coamplification at lower denaturation temperature (COLD)-PCR is a PCR-based application that preferentially enriches the amplification of mutants and variants throughout the course of ...

SuperScript IV UniPrime One-Step RT-PCR System is designed to perform both cDNA synthesis and subsequent PCR amplification in a single tube. This master mix formulation combines the high processivity ...

Invitrogen™ Platinum™ SuperFi II DNA Polymerase is a hot-start, engineered proofreading DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer ...