In ATAC-see, a so-called transposome (2) uses a transposase enzyme (yellow) to insert a pair of fluorescent DNA tags into open regions of chromatin (3). Upon insertion, the DNA is cleaved (4) and the ...

This modification results in a loosening of the coil. Histone modification can open chromatin, thus permitting selective binding of transcription factors that, in turn, recruit RNA polymerase II ...

For instance, histone acetylation reduces the positive charge of the histone proteins, thereby reducing their affinity for DNA and causing the chromatin to open (Figure 2). This in turn permits ...

"This has been a long-standing open question in biology ... developed at Northwestern to individually label and observe chromatin fibers: the bundle of DNA and proteins that form chromosomes ...