While all steps in the IHC protocol affect the quality of the staining outcome, three important factors to consider during initial optimization are primary antibody selection, antigen retrieval, and ...

Every antibody naturally recognizes and binds to particular antigens. The antibodies used in some forms of immunohistochemistry are engineered to bind to antigens involved in cancer. The human ...

An antigen is basically anything on the surface ... A special technique called immunohistochemistry (IHC) is used to identify CD20 and determine whether an abnormal cancerous white blood cell ...

Water-soluble enzymes, antigens and labeling proteins (GFP) are also preserved in-situ, and with appropriate fixation and staining, true localization of enzyme and antigen activity can be visualized.